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Core protein solutions that drive interdisciplinary research and industrial breakthroughs
China’s Perotin × Germany’s NanoTemper: A groundbreaking “protein expression + rapid validation” solution has emerged.
Release date:
2025-03-27
On March 26, 2025, Suzhou PLD Technology Co., Ltd. and Germany’s NanoTemper joined forces to deliver a groundbreaking livestream that shattered the “slow‑paced” paradigm of traditional protein R&D. During this event, the two companies unveiled for the first time their jointly developed Andromeda X—a rapid screening platform that seamlessly integrates cell‑free protein synthesis (CFPS) with spectral shift technology (SpS). This innovative solution catapults protein R&D efficiency into the light speed: from expression and purification to functional validation, the entire workflow is dramatically shortened in both time and cost. It comprehensively addresses key challenges in protein development—low expression efficiency and difficulties in assessing conformational stability—injecting fresh momentum into global biopharmaceutical R&D.
Live Recap: Over 3,000 proteins screened in just 15 minutes—accelerating protein R&D to “highway speed”
1. Cell-Free Protein Expression (CFPS) — A Revolution in Speed and Flexibility
Perotin Bio’s independently developed CFPS technology enables rapid in vitro protein synthesis by harnessing the cell’s intrinsic transcription‑translation machinery. Its key advantages include:
Ultra‑fast synthesis: target protein detected within 1 hour, with peak production achieved in 10 hours (up to 3 mg/mL);
High success rate: For challenging expression sequences such as membrane proteins and toxic proteins, the success rate exceeds 96%;
Flexible compatibility: Supports linear templates, eliminates the need for cloning, and is highly compatible with automated high-throughput screening.
2. Andromeda X Platform — Precise Assessment of Protein Stability and Functional Activity
NanoTemper addresses key pain points in protein expression screening by launching Andromeda X, a cutting-edge platform for rapid protein expression and functional screening. Equipped with highly sensitive Spectral Shift (SpS) technology, it efficiently optimizes recombinant protein expression systems and induction conditions, ensuring both protein stability and functionality. Its core advantages include:
Speed Revolution: 48 samples screened in parallel in just 15 minutes (compared to over 3 hours with conventional methods)
Enhanced information dimension: Integrates expression levels, conformational stability (Ti values), and three-dimensional ligand-binding activity data; requires only 10 μL of unpurified lysate for direct labeling, with no need for concentration or dilution.
Ultra‑sensitive compatibility: Direct detection with 10 μL of lysis buffer, no purification required (supports hosts such as E. coli, HEK293, and Sf9), enabling protein detection at the nM level (remains effective even after 1,000‑fold dilution).
Through a powerful collaboration, Andromeda X has successfully detected the expression of restriction enzymes in cell-free expression system lysates. This cutting-edge protein‑expression screening solution accelerates the entire workflow—from synthesis to validation: after rapid CFPS‑mediated protein expression, Andromeda X immediately assesses folding status, optimizes expression conditions, and verifies protein stability and functional activity, creating a closed‑loop “expression–optimization–validation” process. This approach reduces resource waste associated with futile purification and redundant experiments, significantly enhancing both protein R&D and production efficiency while substantially lowering time and cost.
Act now and seize the future!
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