Technical Service
Develop and produce rare proteins and traditional difficult to produce proteins, achieving high-end "intelligent manufacturing" of drug proteins
Non natural amino acid insertion service
Non-Natural Amino Acids
Site-specific incorporation of non-canonical amino acids endows proteins with novel structures and functions.
Non-natural amino acids (nnAAs)have structurally diverse side chain groups, and inserting them into proteins can endow them with new chemical properties, structures, and biological functions.
PLD Technology relies on its independently developed Cell-free protein expression platform to achieve efficient and rapid synthesis of non natural amino acid inserted proteins. PLD Technology has pioneered non-natural amino acid site-specific insertion services and has successfully completed multi-site nnAA site-specific insertion contracts on a few protein candidates of proteins, antibodies, carriers, etc.
Service Content
With the support of a cell-free system, multi-site insertion is more stable.
pAcF and pAzF targeted insertion
p-acetyl-phenylalanine (pAcF) and para-azido-phenylalanine (pAzF) can be used as the coupling reaction site between antibody and drug to realize the precise control of drug-antibody ratio.
nnAAs insertion test
Based on the aminoacyl tRNA synthetase, tRNA, and nnAAs provided by the customer, PLD Biotechnology provides the testing service using our cell-free platform.
Service Advantages
Endowing proteins with entirely new properties and expanding cutting-edge application scenarios.
A wide range of nnAAs
No transmembrane transport restrictions No cytotoxicity concern
Site-specific insertion
Highly precise insertions of your nnAAs atthe targeted positions
Efficient and fast insertion
Efficient and fast insertion of pAcF,pAzF and other nnAAs
Short service cycle
Application areas
Empowered by non‑natural amino acids, expanding the frontiers of protein applications.
Development of Non-Natural Protein Drugs
The special chemical functional groups of nnAAs expandthe design space of protein drugs, enabling ADCs to havegood pharmacokinetics, potency, and antigen bindingproperties.
Enzyme evolution
The insertion of nnAAs can improve the stability of enzymes and alter their catalytic properties.
Non-natural amino acid probe
nnAAs can be inserted into specific sites of recombinant proteins and serve as highly sensitive biophysical probesfor quantitative detection and precise analysis of the struc-ture and function of target proteins.
New protein materials
The insertion of nnAAs can generate novel protein polymers for the synthesis of biomaterials such as biologi-
cal adhesives
Case Presentation
Stable expression in a cell-free system makes membrane protein purification no longer challenging.
PLD Technology has developed its own CFPS system,where both nnAA and the according orthogonal translation systems are there to achieve site-specific insertion of non natural amino acids into protein sequences.
Figure 1 shows the insertion efficiency of phenylalanine azide(pAzF) and phenylalanine acetyltransferase (pAcF) intomultiple sites of GFP. GFP with nnAAs at multiple sites stillshowed high expression levels;
Figure 2 shows the mass spectrometry analysis results ofwild-type GFP and GFP with pAcF inserted at position 133. Asshown in the figure, the correct insertion rate is extremelyhigh.
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Contact Information
Service Hotline: +86-512-67900128
Customer Service: order@sz-pld.tech
Business Cooperation: bd@sz-pld.tech
Address: Room 302, Building 1, Weili Medical Science and Technology Park, No. 69 Jiepu Road, Suzhou Industrial Park
