Technical Service
Develop and produce rare proteins and traditional difficult to produce proteins, achieving high-end "intelligent manufacturing" of drug proteins
High-throughput, Automated Antibody/Enzyme Synthesis and Screening Services
Industry Pioneer: High-throughput Automated Protein Synthesis and Screening Workstation
Against the backdrop of rapid advances in AI‑driven protein design, antibody discovery, and enzyme engineering, the central challenge facing researchers is no longer simply whether a protein can be expressed, but rather how to accelerate large‑scale functional validation and high‑throughput screening.
Perotin Bio has developed a high-throughput, automated protein‑screening platform based on its proprietary cell‑free protein synthesis (CFPS) technology, offering researchers end-to-end services—from protein sequence design and rapid expression to functional assays—thereby accelerating the translation of research findings from “sequence” to “functional validation.”
Compared with conventional cell-based expression systems, cell-free protein expression eliminates the need for cell culture and complex amplification steps, enabling direct in vitro protein synthesis. This significantly shortens experimental timelines and markedly enhances the efficiency of studying complex proteins, membrane proteins, and recalcitrant proteins.
By integrating an automated liquid-handling workstation with a high-throughput BLI/SPR screening platform, the system enables large-scale parallel expression and functional screening, supporting antibody discovery, enzyme directed evolution, protein–protein interaction studies, and rapid validation of AI‑generated proteins, thereby helping researchers establish a more efficient R&D feedback loop.
Service Advantages
Ultra-fast R&D cycle: It takes only a few hours to generate a functional antibody molecule from linear DNA, representing more than a tenfold increase in R&D efficiency compared with conventional cell-based systems (which require several weeks to months).
High-throughput automation: It screens 3,000 proteins, including antibodies and enzymes, and automates synthesis with no manual intervention, enabling a seamless end-to-end workflow from gene to protein and significantly reducing labor costs.
Open system with flexible adaptability: Reaction conditions (such as pH and temperature) can be freely adjusted, accommodating various antibody formats including VHH, scFv, Fab, and full-length antibodies, with no restrictions on antibody type and support for disulfide bond formation.
Direct detectionThe reaction supernatant can be directly used for ELISA assays, eliminating the time‑consuming purification step and accelerating functional validation and screening; it also supports detection following one‑step magnetic bead‑based purification.
Supports toxic proteins: Does not rely on living cells and can express cytotoxic proteins.
Template preparation: extremely simple and efficient.: Supports linearized DNA fragments as templates
Service Workflow (Taking the Screening of Site-Directed Mutants of Enzymes and Antibodies as an Example)
End-to-end, one-stop membrane protein preparation with controllable timelines and efficient delivery.
Original protein sequence input
Protein sequence analysis and sequence output based on large language models
Automation, high-throughput Cell-free protein expression
Based on Perotin Bio’s fully autonomous cell-free protein expression technology and a fully automated high-throughput workstation, the sample throughput exceeds 1,000 protein samples per day.
High-throughput BLI/SPR screening
High-throughput label-free screening based on biomolecular interference/surface plasmon resonance
Project Workflow
Synthesis of the Original Plasmid Gene
Testing of the native enzyme, antibody expression, and activity assay protocols
Construction of Mutant Genes
High-Throughput Protein Expression and Activity Screening
Data Delivery
Specific content
Original gene sequence prior to synthetic mutation (customers may provide it directly)
After rapidly expressing the native enzyme and antibody sequences, validate the activity assay protocol using the supernatant or crude purified fraction.
Introduce mutations into the target sequence using primer-based PCR.
CFPS, in conjunction with an automated liquid handling workstation, offers high-throughput automated protein expression and activity screening services.
Mutant activity ranking and sequencing results of top-ranked mutants
Cycle
5–10 business days
1–5 business days
1–2 business days
1–5 business days
1–5 business days
Video Display
CFPS Enables Fully Automated High-Throughput Protein Expression and Screening
Case Showcase
Case Study 1: High-throughput CFPS and Screening based on Mutant Library—phi29 DNA Polymerase
By establishing a CFPS high-throughput screening and validation of mutant enzymes, PLD completed the expression and assay of 96 phi29 DNA polymerase mutants in 3 days, identifying high-efficiency variants. This approach drastically reduced screening time and labor costs, and greatly improved the R&D efficiency.
Case Study 2: High-throughput screening model for targeted mutant GFP
Used on the high-throughput automation workstation, PLD's CFPS platform quickly achieved mutant protein synthesis and screening involving each of the five sites in the active center of the green fluorescent protein GFP.
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Contact Information
Service Hotline: +86-512-67900128
Customer Service: order@sz-pld.tech
Business Cooperation: bd@sz-pld.tech
Address: Room 302, Building 1, Weili Medical Science and Technology Park, No. 69 Jiepu Road, Suzhou Industrial Park
