Technical Service
Develop and produce rare proteins and traditional difficult to produce proteins, achieving high-end "intelligent manufacturing" of drug proteins
Membrane Protein Expression Services
Membrane protein
Professional membrane protein expression services, with high success rates and excellent stability.
As complex functional proteins, membrane proteins play an important role in material transport, signal transduction, energy conversion and cell differentiation. Membrane proteins face challenges during expression, purification and structural analysis.
PLD Cell Free Protein Synthesis system can maintain the stability of membrane protein，by incorporating detergent or nanodisc into the expression system, making it easier to express and purify membrane proteins.
Success rate of our membrane protein expression is close to 100%.The membrane proteins that have been successfully expressed include cytochrome enzyme, respiratory chain enzymes, ligand receptors, etc.
Detergent-Supplemented Cell-Free Platform (No Expression No Charge)
Proprietary core technologies to establish a native hydrophobic environment for membrane proteins.
Leveraging its proprietary CFPS technology, PLD Biotechnology achieves soluble expression of full-length transmembrane proteins by directly supplementing detergents into the cell-free system. These detergents provide a hydrophobic environment for the transmembrane domains of membrane proteins, facilitating their proper folding into native conformations.
Nanodisc-Supplemented Cell-Free Platform
Cell-free + nanodisc dual technology for stable expression of highly active membrane proteins.
By supplementing membrane scaffold proteins and phospholipids into the cell-free system, PLD Biotechnology enables the self-assembly of nanodiscs that mimic native cellular membranes. Synthesized membrane proteins directly incorporate into these nanodiscs, with their hydrophobic transmembrane domains stably embedded within the lipid bilayer. This platform yields full-length membrane proteins with enhanced stability, functional activity, and near-native conformations.
Case Showcase
CXCR4 (seven-transmembrane)
CXCR4 can be expressed in a soluble form directly in the supernatant by adding detergent or nanodiscs to the system.
SomeSeven-transmembrane proteinBy adding a detergent to the system, soluble expression can be achieved directly in the supernatant.
CLDN18.2 (tetraspanin)
CLDN18.2 is directly expressed in soluble form in the supernatant by incorporating nanodiscs into the system.
SomeTetraspanin membrane proteinBy adding a detergent to the system, soluble expression can be achieved directly in the supernatant.
Service Advantages
Customized Systems, High Purity, Rapid Delivery — A One-Stop Solution for Efficient Membrane Protein Expression
System Optimization
Establish distinct in vitro reaction systems tailored to the characteristics of different proteins.
High purity
Membrane protein purity ~80%, with endotoxin levels as low as 1 EU/µg.
Efficient and rapid synthesis
Protein delivery in as little as 3 days
Service Process
End-to-end, one-stop membrane protein preparation with controllable timelines and efficient delivery.
Project Workflow
Preparation of Gene Synthesis and Expression Templates
Preliminary Expression and Optimization
Protein overexpression
Purified protein
Specific content
Codon optimization, full‑gene synthesis, and PCR amplification of the expression template
Screening multiple detergent/nanodisc conditions
Based on demand and production estimates
Endotoxin levels are as low as 1 EU/μg; membrane protein content is approximately 80%.
Cycle
5–10 business days
1–5 business days
1–5 business days
1–10 business days
Delivery Content
Complete delivery, worry-free and hassle-free.
Plasmid of interest (2 µg)
Protein Expression and Purification Experimental Report (Experimental Protocol and Results)
Purpose: Membrane protein product (purity ~80%, endotoxin 1 EU/µg)
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Contact Information
Service Hotline: +86-512-67900128
Customer Service: order@sz-pld.tech
Business Cooperation: bd@sz-pld.tech
Address: Room 302, Building 1, Weili Medical Science and Technology Park, No. 69 Jiepu Road, Suzhou Industrial Park
