Technical Service
Develop and produce rare proteins and traditional difficult to produce proteins, achieving high-end "intelligent manufacturing" of drug proteins
Expression Service of Hard-to-express Proteins
Cell-free system
Cell-free in vitro protein synthesis efficiently overcomes the challenge of expressing proteins that are difficult to produce in cells.
Cell-Free Protein Synthesis (CFPS)does not rely on living cells and directly synthesizes proteins in vitro. Relying on its independently developed cell-free protein expression platform, PLD Technology achieved efficient and rapid synthesis of proteins that are difficult to express in cells (such as membrane proteins), becoming a savior for difficult-to-express proteins.
Service Advantages
Backed by cutting-edge technology and backed by attentive customer service.
Success rate higher than
91 protein sequences were randomly selected and the protein expression success rate was as high as 96% using the PLD
cell-free protein expression system (whether the plasmid framework is suitable has been considered).
Fastest delivery time
If you could provide template DNA
Varieties of protein
Non-natural proteins and toxicproteins can be synthesized
No expression, no charge.
Gene synthesis fee is chargedseparately
Service Process
Standardized processes for fast and reliable delivery.
Sequence Optimization and Synthesis
5–10 business days
Molecular Construction
3–5 business days
Preliminary Expression and Optimization
1–2 business days
Protein overexpression
3–5 business days
Purified protein
5–10 business days
Delivery Content
Complete delivery, worry-free and hassle-free.
Target plasmid (2 ug)
Protein expression purification experiment report (test protocol and results)
Target protein product (purity ~90%, endotoxin 1EU/ug)
Examples of Partially Successfully Expressed Proteins
Stable expression in cell-free systems overcomes the bottleneck of difficult-to-express proteins.
Service Advantages
One-stop, high-efficiency protein expression solution
System Optimization
Construct distinct in vitro reaction systems tailored to the characteristics of different proteins.
High purity
Recombinant protein product (purity 80% or 90%, endotoxin content as low as 1 EU/µg)
Efficient and rapid synthesis
Protein delivery in as little as 3 days
Service Process
End-to-end, one-stop preparation of challenging proteins, with controllable timelines and efficient delivery.
Project Workflow
Preparation of Gene Synthesis and Expression Templates
Preliminary Expression and Optimization
Protein overexpression
Purified protein
Specific content
Codon optimization, full‑gene synthesis, and PCR amplification of the expression template
Screening multiple detergent/nanodisc conditions
Based on demand and production estimates
Endotoxin levels are as low as 1 EU/μg; membrane protein content is approximately 80%.
Cycle
5–10 business days
1–5 business days
1–5 business days
1–10 business days
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Contact Information
Service Hotline: +86-512-67900128
Customer Service: order@sz-pld.tech
Business Cooperation: bd@sz-pld.tech
Address: Room 302, Building 1, Weili Medical Science and Technology Park, No. 69 Jiepu Road, Suzhou Industrial Park
