Cell-free protein expression kit-PLD_biomedical

Free opportunity to “break the membrane”! Custom membrane protein expression services—available for a limited time; don’t miss out!

order@sz-pld.tech

+86-512-67900128

Order

language

Product Center

Home

Cell-free protein expression kit

Product Overview

Are you tired of spending days on end performing conversions and painstaking cell culture to obtain the protein you’re interested in? In today’s fast-paced world, where time is money, are you looking for a cost-effective protein production platform? Compared with traditional cell-based methods,Cell-free protein expressionIt accelerates your research and development.High-quality solution, enabling you to reduce and control costs while flexibly optimizing reaction parameters, thereby obtaining your target protein product more quickly and efficiently.

“The purified proteins produced by cell-free protein expression technology surpass conventional methods in both quantity and speed. Each expressed protein can serve as a therapeutic candidate, enabling us to generate data packages two months faster than before while also reducing costs. By continuing to leverage cell-free protein expression, we anticipate saving approximately six months of a full-time employee’s time over the next 18 months.”

— Evaluations of Cell-Free Protein Expression Technology by International Clients

The PLD cell-free protein expression kit relies on T7 transcription and the intrinsic translation machinery of E. coli. The reaction system contains all the biological components required for transcription, translation, protein folding, and energy regeneration, including ribosomes, tRNAs, molecular chaperones, metabolic enzymes, and translation initiation and elongation factors. The kit can use PCR products or circular plasmids as templates; simply add your template to initiate the reaction.Rapid and efficient protein expression.

Product Features

Quick

The target protein can be detected in as little as 1 hour, and protein synthesis reaches its peak within 10 hours.

Template flexibility

Supports plasmid templates as well as PCR templates*;

High expression level

Protein preparation with a yield of up to 3 mg/mL or higher;

Applicable to a wide variety of proteins.

It can express various types of proteins, including those rich in disulfide bonds and membrane proteins.

Easy to operate

In a one-step reaction, the protein can be prepared simply by mixing the reaction components with the DNA template.

High throughput

Expression screening can be performed in a 96-well plate, and once positive expression is detected, the reaction scale can be scaled up.

Protein co-expression

It is possible to co-express multiple proteins in the same reaction;

Lower cost

Reduce labor costs

Product Composition

The PLD cell-free protein expression kit provides a method for preparing recombinant proteins, enabling easy detection and purification. The expression system provided by this kit primarily includes:

Optimized Escherichia coli extract

It enhances the structural stability during DNA transcription and translation, thereby increasing the yield of soluble proteins.

Optimized reaction buffer

It continuously supplies energy for protein synthesis via the ATP regeneration system.

Amino acids with optimized concentration ratios

Provide an adequate supply of substrates for protein synthesis;

Optimized expression vector containing the GFP gene

GFP serves as a positive control, allowing for the direct visualization of protein expression results.

Product Applications

The PLD cell-free protein expression kit can be used in, but is not limited to, the following applications:

① Antibody Discovery and Validation

② High-throughput screening

With the PLD cell-free protein expression kit, you can go from a DNA sequence to an antibody in the shortest possible time.1–2 daysYou can obtain target antibodies in quantities ranging from 100 μg to several milligrams, saving you valuable time and accelerating your R&D process.

Case Data

Product Performance Demonstration of the PLD Cell-Free Protein Expression Kit

Figure 1A

Figure 1B

PLD Cell-Free Protein Expression KitIt exhibits excellent protein expression performance,Protein yield can reach as high as 3–5 mg/mL.After mixing the kit components with commonly used fluorescent protein plasmids and incubating at 30°C, fluorescence emitted by a large number of fluorescent proteins can be observed without UV irradiation (Figure 1A). This product exhibits excellent batch-to-batch reproducibility and stability. Under standard laboratory conditions, linear scale-up from the μL to the mL range is readily achievable (Figure 1B).

Product Performance

Reagent model

PLDK001

PLDK002

PLDK003

PLD-AB01

HTE01

Linear template support

√

Disulfide bond support

√

Yield