Expression Service of Hard-to-express Proteins

Cell-free system

Cell-Free Protein Synthesis (CFPS) does not rely on living cells and directly synthesizes proteins in vitro. Relying on its independently developed cell-free protein expression platform, PLD Technology achieved efficient and rapid synthesis of proteins that are difficult to express in cells (such as membrane proteins), becoming a savior for difficult-to-express proteins.

Service Advantages

Service Process

Deliverables

Target plasmid (2 ug)
Protein expression purification experiment report (test protocol and results)
Target protein product (purity ~90%, endotoxin 1EU/ug)

Some successful protein expression cases

The proteins synthesised by the cell-free expression system have comparable or even better protein activities than other prokaryotic and eukaryotic expression platforms. Taking IL-2 as an example, the IL-2 activity of PLD Technology is comparable to that of an imported brand of IL-2 expressed in CHO cells, and also better than that of a domestic brand of prokaryotic expression product.

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Cell-free expression of IL-2 vs. other brands of IL-2 activity

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Cell-free expression of antibody electrophoresis and ELISA binding activity

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Electrophoresis of some successfully expressed proteins

Recombinant protein

Interleukins, interferons, tumour necrosis factors, colony-stimulating factors, chemokines, growth factors, etc.	Various biologically active proteases.	G protein-coupled receptors, water channel proteins, ion channel proteins, etc.

Antibody-like proteins

Full length lgG	Fab	ScFv/VHH	Nucleic acid polymerase (NP)
Trastuzumab	Ranibizumab	Brolucizumab	Phi29 DNA polymerase
Toripalimab	Cetuximab	Caplacizumab	Terminal deoxyribonucleic acid transferase (TdT)
Metabolic key enzyme	Membrane Proteins	Special Target Proteins	Hard-to-express proteins
Caspase-1	Water channel protein	Calreticulin	Leopard frog enzyme
3-Phosphoglycerol dehydrogenase	CXCR4	Botulinum toxin Hc segment	Snake venom serine protease
Restriction endonuclease	Interleukin	Cytokines	Pharmaceutical Protein Ingredients
EcORI	IL-2	IFN2	Ustatin
HindlII	IL-4	GM-CSF	Reteplase
Smal	IL-7	VEGF	Transpeptidase
BamHl	IL-15	EGF	Cyanobacterial antiviral protein N
Xbal	IL-21	wnt3a	Heme oxygenase